| Protein name |
Beta-galactosidase |
| Host compatibility |
Kluyveromyces lactis (non-GM) |
| Yield |
Non-GM K. lactis: Not reported in g/L, 0.12 U/mL (Rodríguez et al., 2006)
|
| Scale (in specific example) |
Lab scale in flask (Rodríguez et al., 2006)*
|
| Biggest scale product has been develloped in this organism |
Industrial (DSM) |
| Regulatory status in Europe |
EFSA approved beta-galactosidase from the the non-GMO K. Lactis strain used by DSM (Lambré et al., 2022b). Production by GM K.lactis is not allowed
|
| Regulatory status in other parts of the world |
FDA GRAS approval in US. Not allowed in Canada
|
| Companies producing this protein in this organism |
DSM (both GMO and non-GMO)
|
| Extra/remark |
|
| Publications/references |
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Rodríguez, Á. P., Leiro, R. F., Trillo, M. C., Cerdán, M. E., Siso, M. I. G., & Becerra, M. (2006). Secretion and properties of a hybrid Kluyveromyces lactis-Aspergillus niger β-galactosidase. Microbial Cell Factories, 5(1). https://doi.org/10.1186/1475-2859-5-41
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Lambré, C., Baviera, J. M. B., Bolognesi, C., Cocconcelli, P. S., Crebelli, R., Gott, D. M., Grob, K., Lampi, E., Mengelers, M., Mortensen, A., Rivière, G., Steffensen, I., Tlustos, C., Van Loveren, H., Vernis, L., Zorn, H., Glandorf, B., Lunardi, S., Liu, Y., . . . Chesson, A. (2022b). Safety evaluation of the food enzyme β‐galactosidase from the non‐genetically modified Kluyveromyces lactis strain GAL. EFSA Journal, 20(10). https://doi.org/10.2903/j.efsa.2022.7574
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Meena, M., Zehra, A., Dubey, M. K., Aamir, M., & Upadhyay, R. S. (2018). Penicillium Enzymes for the Food Industries. In Elsevier eBooks (pp. 167–186). https://doi.org/10.1016/b978-0-444-63501-3.00009-0
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Fernandes, P. (2018). Enzymatic Processing in the Food Industry. In Elsevier eBooks. https://doi.org/10.1016/b978-0-08-100596-5.22341-x
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Saqib, S., Akram, A., Halim, S. A., & Tassaduq, R. (2017). Sources of β-galactosidase and its applications in food industry. 3 Biotech, 7(1). https://doi.org/10.1007/s13205-017-0645-5
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Bechtel, A., Seitl, I., Pross, E., Hetzel, F., Keutgen, M., & Fischer, L. (2024). Recombinant production of Paenibacillus wynnii β-galactosidase with Komagataella phaffii. Microbial Cell Factories, 23(1). https://doi.org/10.1186/s12934-024-02544-5
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Movahedpour, A., Ahmadi, N., Ghalamfarsa, F., Ghesmati, Z., Khalifeh, M., Maleksabet, A., Shabaninejad, Z., Taheri‐Anganeh, M., & Savardashtaki, A. (2021). β‐Galactosidase: From its source and applications to its recombinant form. Biotechnology and Applied Biochemistry, 69(2), 612–628. https://doi.org/10.1002/bab.2137
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Kaushal, J., Singh, G., & Arya, S. K. (2022). Emerging trends and future prospective in enzyme technology. In Elsevier eBooks (pp. 491–503). https://doi.org/10.1016/b978-0-323-89929-1.00036-6
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Chen, W., Chen, H., Xia, Y., Zhao, J., Tian, F., & Zhang, H. (2008). Production, Purification, and Characterization of a Potential Thermostable Galactosidase for Milk Lactose Hydrolysis from Bacillus stearothermophilus. Journal of Dairy Science, 91(5), 1751–1758. https://doi.org/10.3168/jds.2007-617
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